Pages: (92-116 )
Abstract
The aim of this study was to evaluate and compare the effect(s) of the “Gbogbonise Epa Ijebu 200 cure” herbal remedy on some haematological parameters in male wistar rats infected with Salmonella typhi. Sixty male rats weighing 120-150g were randomly divided into 10 groups of 6 rats each (n=6). Group 1, 2, 3, 7, 8, 9 and 10 received one (1) ml of Salmonellae typhi suspension with a concentration
of 106 CFU/mL in phosphate-buffered saline (PBS), while Group 4, 5 and 6 served as the Zero control, undiluted herbal control and diluted herbal control, respectively. Day 8 post-infection, serological assay and stool culture were carried out to confirm Salmonella typhi infection. Rats in Group 2 and 3 were not treated with the herbal remedy but with Ciprofloxacin and phosphate-buffered saline (PBS), respectively, which serve as the positive and negative control, respectively. Meanwhile, rats in group 7 and 8 were treated with 500mg/kg/bid and 250mg/kg/bid herbal remedy singly, respectively; while groups 9 and 10 were treated with the equal mixture of the Ciprofloxacin and herbal remedy, administered orally twice daily for seven days. At days 8 and 16 post-treatment, rats from each group were sacrificed to collect their blood sample to determine the effect of the herbal remedy on some haematological parameters. Data generated were analyzed using SPSS Version-20. At post-treatment, rats in groups 7, 8, 9 and 10 had a significant (P<0.001) higher mean RBC count, PCV, Hb, MCH, MCV and
MCHC, as well as lower mean platelets count WBC and absolute neutrophils count when compared to the S. typhi-infected control group (Group 1). Meanwhile, the lymphocytes count increased nonsignificantly. The outcome of this study shows that Gbogbonise Epa Ijebu 200 cure herbal remedy, when tested singly and in combination with Ciprofloxacin, reversed and ameliorated the haematotoxic effects induced by S. typhi infection in the rats.
Keywords: Gbogbonise Epa Ijebu 200 Cure, Salmonella typhi, Heamatological parameters, Rats,