Pages: (17-25 )
Abstract
Plant tissue culture is an aspect of biotechnology devoted to laboratory methods of propagation and obtaining pathogen-free plants. Many types available include embryo culture with which success have been obtained with many herbaceous and tree crops. Using basal Murashige and Skoog (MS) medium and regulating the constituents and concentration of Auxins and Cytokinins as growth regulators, embryo of Parkia biglobosa was successfully cultured in vitro. This research demonstrated that P. biglobosa embryo culture does not require any specialized growth medium, nevertheless optimum success was obtained using the basal Murashige and Skoog (MS) medium with 6- benzylaminopurine (BAP) (0.5mg L-1) and 3-indole butyric acid (IBA) (0.2mg L-1). This provided the opportunity to obtain pathogen-free clones and large scale micropropagation of this economic crop. The success of the operation was due to effective disinfection of plant materials and equipment, good media preparation and careful culture initiation. This research work shows potential in vitro regeneration, propagation and preservation of P. biglobosa which are better alternatives to conventional vegetative propagation.
Keywords: : BAP (6- benzylaminopurine), IBA (3-indole butyric acid), MS medium, Parkia biglobosa, Tissue culture.,