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Abstract
The increase in multidrug resident pathogens is of public health concern.This study is aimed at detecting and identifying the ESBL SHV, TEM and CTX-M resistant genes from Gram-negative urinary pathogens isolated from selected hospitals in Ogun State Nigeria. Ninety seven Gram-negative bacilli isolates from UTI patients were obtained from FMC and OOUTH. They were sub-cultured, Gram-stained and identified biochemically. Antibiotic susceptibility testing was performed on all the pathogens using Kirby-Bauer’s method. Molecular testing was done using PCR for the detection of BLA-SHV, BLA-TEM and BLA-CTX-M genes. One-way analysis of variance (ANOVA) was used to examine the data using the Statistical Packages for Social Scientists SPSS-21.0 (version 21.0). It compared means relative to the variance between and within groups. Statistical significant difference was considered at p-value of P≤ 0.5. The distribution of the isolates obtained among the two hospitals includes: 49 (50.5%) in FMC and 48 (49.5%) in OOUTH. The Isolates were identified biochemically as Escherichia coli 50 (51.5%), Klebsiella spp. 17 (17.5%) and Proteus spp. 30 (30.9%). E. coli and Klebsiella spp. had the highest resistance of 97.9% to Cefotaxime and Nalidixic acid. E coli also had complete resistance to Nalidixic acid, Cefuroxime, Zemifloxacin and Augmentin. All Klebsiella species isolated from both hospitals showed complete resistance to Augmentin, Cefotaxime and Cefixime. Proteus showed the highest resistance to Augmentin among other antibiotics. All the isolates were multidrug resistant and they were tested for ESBL resistant genes using Molecular Techniques. Out of the 97 organisms, 2 (2.1%) were positive for the TEM gene, CTX-M gene (21.6%) was detected among the isolates. There was no SHV gene detected in the isolates that were tested. The findings highlighted the significant resistance of Gram-negative bacilli to various antibiotics.
Keywords: Cefotaxime-hydrolyzing β-lactamase,Sulf-hydryl variable and Temoneria, Antibiotic resistance, Enterobacteriacaea, Extended Spectum Beta-lactamase,