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Abstract
In recent years there have been an advancement in the study of the antimicrobial activities of nanoparticles synthesized using medicinal plant. In this study, the seed and leaf extracts of Jatropha curcas was used in the biosynthesis of silver (Ag) nanoparticles. The goals of this study are to compare the antibacterial properties of seed and leaf biosynthesized silver (Ag), and to also characterize them using Scanning electron microscope (SEM), Transmission electron microscope (TEM), X-ray Diffraction (XRD), Fourier transformed infra-red spectroscopy (FTIR) and UV spectroscopy. XRD studies verify the cubic crystal structure and average diameters of Ag to be 13.3 nm for nano seeds, while for the nano leaf the average diameter was 14.31 nm. The spherical morphologies nano-dimensions of 40 nm and 50 nm were verified by scanning electron microscopy for AgNPs of the leaf and seed respectively. The EDX analysis confirmed that the AgNPs seed consist of 66.24% weight for Ag and 60.96% weight for Ag in the leaf. The highest zone of inhibition of 30mm was recorded against S. aureus (ATCC25923), E. coli and S. pyogenes by the silver nanoparticles mediated from the leaf at 100µg/ml. and the seed was able to inhibit the organisms at various concentrations. In conclusion this research study showed that Jatropha curcas extract, for both the seeds and leaf, has a good potential for the biosynthesis of nanoparticle, However, the J. carcus synthesized with the leaf showed better antimicrobial activities compared to the seed, which thus has the possibility of using the plant extract for therapeutic drug in treating multidrug resistance infections.
Keywords: Antibacterial, Biosynthesis, Jatropha curcas, Silver, X-ray Diffraction (XRD),